Improved synthesis of 3-14C-3-hydroxy-3-methylglutaryl coenzyme A.
نویسندگان
چکیده
منابع مشابه
Improved synthesis of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA).
An improved method for the chemical synthesis of HMG-CoA is described. The procedure is designed to prevent the formation of 3-acetoxy-HMG-CoA.
متن کاملPurification of 3-hydroxy-3-methylglutaryl coenzyme A reductase.
The enzyme 3-hydroxy-3-methylglutaryl coenzyme agarose, glucose-6-phosphate dehydrogenase, and aldolA reductase from human liver was purified to homogeneity. ase were from Sigma Chemical co.; agarose-hexaneHMG-CoA was from P-L Biochemicals; bovine serum Electrophoresis of the purified human enzyme on non-denaturing gels revealed a single protein-staining band that co-migrated with reductase act...
متن کاملClass II 3-hydroxy-3-methylglutaryl coenzyme A reductases.
The biosynthesis of isopentenyl diphosphate (isopentenyl pyrophosphate), the precursor of isoprenoids in all forms of life, occurs by two distinct metabolic pathways, the mevalonate pathway (Fig. 1) and the glyceraldehyde 3-phosphate/pyruvate pathway, often termed the nonmevalonate pathway (17). Whereas many gram-negative bacteria employ the nonmevalonate pathway (26), humans, other eukaryotes,...
متن کاملCytoplasmic 3-Hydroxy-3-methylglutaryl Coenzyme A Synthase from Liver
The cytoplasmic fraction of avian liver contains multiple forms of 3-hydroxyd-methylglutaryl coenzyme A synthase which are distinct from their mitochondrial counterpart. Of the four cytoplasmic species, synthases I and II predominate, constituting 22 and 62%, respectively, of the total cytoplasmic synthase activity; synthases III and IV are minor forms comprising the remaining 16%. Each of the ...
متن کاملImproved methods for the assay and activation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.
A simple and rapid mixed-phase method for the quantitative assay of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase and a procedure for the efficient reactivation of Mg-ATP-inactivated microsomal HMG-CoA reductase by potato acid phosphatase are described. The mixed-phase assay entails the direct addition of the acidified, deproteinized incubation mixture to a toluene-based scintillation fluor. T...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1975
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.39.1675